Neuroprotective role of chrysin in attenuating loss of dopaminergic neurons and improving motor, learning and memory functions in rats

  • Mohammad Rashid Ahmed Baqai Medical University, Karachi
  • Masood Ahmed Shaikh College of Medicine, Qassim university, SaudiArabiaBaqai Medical University, Karachi Pakistan
  • Syed Imran Ul Haq Dow University of Health sciences, Karachi
  • Shakila Nazir Baqai Dental College Karachi
Keywords: chrysin, immuno-histochemsitry, motor behavior, Parkinson disease, rotenone

Abstract

Objective: Selective degeneration of dopaminergic neurons is the pathological hallmark of Parkinson disease (PD). Enhanced oxidative stress, lipid peroxidation and susceptibility of dopaminergic neurons to apoptotic cellular death are the leading pathogenetic mechanisms. Chrysin is an active flavonoid. Its neuroprotective effects have been reported. This study examined the neuroprotective effects of chrysin in ameliorating the dopaminergic neuronal degeneration and motor behavioral changes in rotenone model of PD.

Methods: Thirty Sprague-Dawley rats were assigned into three groups: Control, rotenone-treated, and rotenone+chrysin treated groups. Rotenone was given at a dose of 3 mg/kg daily intraperitoneally, and chrysin was given at a dose of 50 mg/kg daily intraperitoneally for 4 weeks. Using five neurobehavioral assessment tests, evaluation was done weekly to record the motor behavioral changes. After 4 weeks, animals were sacrificed, brains were removed, and section from striatum and substantia nigra were stained using hematoxylin and eosin and cresyl violet stains. Immunohistochemical sections were also prepared using anti-tyrosine hydroxylase (TH) antibody.

Results: Rotenone-induced Parkinson like changes were evident from deteriorating motor behavior. These animals showed extensive loss of dopaminergic neurons, decreased immunoreactivity against anti-TH antibodies and number of TH positive dopaminergic neurons in the nigrostriatal region. Chrysin treated animals showed a significant reduction in motor behavioral changes, degeneration and loss of nigrostriatal dopaminergic neurons and increased immunoreactivity to anti-TH antibody.

Conclusion: This study concludes that chrysin confers neuroprotection in rat model of PD. It attenuates the degeneration of the nigrostriatal dopaminergic neurons and motor behavioral abnormalities. 

Author Biographies

Mohammad Rashid Ahmed, Baqai Medical University, Karachi
Associate Professor, Department of Anatomy, Baqai Medical college Karachi Pakistan
Masood Ahmed Shaikh, College of Medicine, Qassim university, SaudiArabiaBaqai Medical University, Karachi Pakistan

PROFESSOR AT DEPARTMENT OF ANATOMY AND HISTOLOGY,

Syed Imran Ul Haq, Dow University of Health sciences, Karachi

Assistant professor, Department of Pharmacology

Shakila Nazir, Baqai Dental College Karachi
Professor, Department of Oral Biology
Published
2018-04-19
How to Cite
Ahmed, M., Shaikh, M., Ul Haq, S., & Nazir, S. (2018). Neuroprotective role of chrysin in attenuating loss of dopaminergic neurons and improving motor, learning and memory functions in rats. International Journal of Health Sciences, 12(3). Retrieved from https://ijhs.org.sa/index.php/journal/article/view/2433
Section
Original Paper